I have prepared the conidial suspension of fungi and now I want to adjust the spore concentration by hemocytometer> Your suggestions are needed.
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Hi Aqleem
You can read this "Reference1"
https://cals.arizona.edu/classes/plp427L/lab2.html
Also "Reference2"
http://crcooper01.people.ysu.edu/Using%20a%20Counting%20Chamber%20090709.pdf
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Also "Reference4"
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3779437/
And "Reference6"
https://link.springer.com/chapter/10.1007/978-981-10-0021-8_12/fulltext.html
Several good references here on ResearchGate.
Check this discussion on a 5x10(4) spore concentration:
Note the answer from: Susann Auer · - · Technische Universität Dresden:
"Since you obviously have to adjust your spores to an exact concentration I recommend that you count as many squares as possible and repeat your counting with another sample of your spore suspension. So you will assess proper mixing of the sample prior to counting.:
How to find the find the concentration of fungal spores? - ResearchGate. https://www.researchgate.net/post/How_to_find_the_find_the_concentration_of_fungal_spores2 [accessed Feb 7, 2017].
Also:
https://en.wikipedia.org/wiki/Hemocytometer#Usage
https://www.researchgate.net/post/How_to_prepare_a_fungal_spore_suspension_from_a_plate_or_tube_for_later_inoculation_into_liquid_media
https://www.researchgate.net/post/How_to_find_the_find_the_concentration_of_fungal_spores2
https://www.researchgate.net/post/How_to_perform_cell_counting_bacteria_using_hemocytometer
www.uky.edu/Ag/Wheat/wheat_breeding/protocols/Using%20Hemacytometer.doc
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