The TiO2 are synthesized by sol-gel process and they are 8 nm in particle size.
Loading of the Silver Nitrate AgNO3 (50mg/l) to the TiO2-NP, this can improve the Antifungal activity of TiO2-NP.
The most basic way would be to expose TiO2 nanoparticles to sunlight or to simulated sunlight. TiO2 will become photoactivated and it will display higher anti fungal effect compared to non-photoactivated TiO2.
Aantifungal and photocatalytic properties of TiO2 NPs have been well known for many years. Aantifungal activity of TiO2 NPs is enhanced on exposure to UV light
.On surface modification of TiO2 NPs with the deposition of metals like Ag,Au,Pt ,Cu,Fe,…. These metals are used as active agents in coatings TiO2 NPs to produce antifungal surfaces. Also, deposition of metals may causes to shift the absorption edge of TiO2 NPs to higher wavelength . TiO2 NPs concentration is an important factor in it's antifungal activity.
Dear researchers:
Thank you for your answers. I have to tell you that we have tried with the light activation of the TiO2 nanoparticles, We found no difference in the inhibition of bacteria.
The experiments that we performed were:
1. Solution of TiO2 nanoparticles in stirring behind UV light during 20 min, 90 min and 120 min. .
2. We tried the same but without stirring.
Do you have the protocol for this activation process??
Hi,
you can read this article: DOI: 10.1016/j.ceramint.2013.11.013.
We have participated on similar experiment involving C. Albicans and TiO2 where the %SR of yeast was reduced to below 20% in 60minutes. There is also a protocol of such process.
Basically, you make a suspension of yeast and powder, then irradiate it with Wood's lamp for 60min and count number of CFUs.
One more thing - the phase and crystallinity (our best for C.A. was 38nm) of TiO2 is very important; anatase exhibits photocatalytical activity under UV-A radiation.
Dear Robert Michal, thank you for your answer. We will read your suggested paper.
We are using anatase crystalline phase of the TiO2.
Best regards.
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