Dear all,
I am currently trying to find the concentration of RNA present in a solution in terms of copies of RNA/volume of solution. The RNA molecules are 508 bases in size. I will be using the formula involving Avogadro's constant and the concentration of RNA in terms of ng/ul to find the concentration in terms of copies of RNA/volume of solution. The RNA strands are all + sense strands. Although the RNA molecules are not complementary, I'm not sure if they could potentially form regions of dsRNA.
How can I know if my RNA is single stranded or double stranded in solution? This is important since dsRNA would require me to multiply the molar mass of the ssRNA by 2, which can cause the concentration in copies of RNA/volume of solution to differ greatly.
Thank you!
Lots of online tools to help with your question. If they are all + strands, then you don't need to worry about the single or double stranded part of your question.
http://www.endmemo.com/bio/dnacopynum.php
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