Nathanael Quake

5 Questions 4 Answers 0 Followers

Questions related from Nathanael Quake

Unusual qPCR Amplification Curves?

Hi all, May I have some suggested reasons why I am obtaining amplification curves (in pink) with unusual shapes (particularly near the baseline) and plateaus of various heights? This is a...

26 December 2019 9,414 3 View

Choice of Statistical Test for Groups with Different Normalities?

Hi all, If I wish to compare data from two groups, one of which has data that is normally distributed and the other which has data that is not normally distributed should a parametric or...

16 February 2018 6,030 3 View

How can I accurately determine RNA concentration based on molar mass?

Dear all, I am currently trying to find the concentration of RNA present in a solution in terms of copies of RNA/volume of solution. The RNA molecules are 508 bases in size. I will be using the...

11 October 2016 2,891 1 View

Why do I have poor DNA quality and yield after gel extraction and clean up?

Dear all, I amplified a sequence from a plasmid template. The amplicons will serve as templates for IVT. The amplicon is 500bp in size, while the plasmid is about 2000bp in size. I do not wish to...

06 October 2016 6,998 9 View

Is a PCR efficiency of 95% to 105% essential for qPCR?

Hi all, I am currently using qPCR extensively, and I have some questions regarding relative quantification and PCR efficiency. If I use the Livak method for relative quantification, does it...

22 September 2016 1,464 5 View