When isolating gastric gland from mouse stomach, most protocol from published study only mention separating the 2 layers before isolating the gland, but do not specify exactly how.
Does it involve scraping the inner layer from the outer muscular layer?
How can one tell the mucosal layer has been scraped off?
May try EDTA to isolate fractions of intestinal cells. Cano-Gauci DF et al. Experimental Cell Research. 1993;208:344-349.
If you are not interested in keeping the structure you may try scraping with a porta (e.g. for PCR ). Afterwards, if you wanna make sure there is no mucosa left on the muscular layer, you can make a swis roll of the muscular layer and fix it. You can process it for IHQ and do PGP9.5. If the submucosa has been removed succesfully, you shouldn't see the submucosal plexus.
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