Fatima Ahmed

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Questions related from Fatima Ahmed

How can fragmented RNA which is extracted from FFPE tissue sample be transcribed into cDNA most effectively? Which kit works the best in this case?

Older FFPE tissues are used for RNA extraction by Trizol based method which is then transcribed into CDNA using a kit, having a lower concentration and purity. Thermo Scientific kit is used for...

06 May 2025 3,038 0 View

I would like to ask about most suitable method of RNA extraction from FFPE tissues of breast cancer patients and expression of house keeping genes?

Which method is more suitable for RNA extraction from FFPE tissues from breast cancer patients, since Trizol-based method is giving lower absorbance while kit-based method is lowering the conc....

17 April 2025 5,143 0 View