How can enzyme activity results from sphectrophotometric assay be corrected to take into account the absorbance by biochar?

Muhammad Farid Azlan Halmi @Muhammad-Farid-Azlan-Halmi

06 August 2017 1 3K Report

I realize that some authors used different standard curves in spectrometeric/ colorimetric enzyme assay in order to account for the adsorption that some of the biochar had on the product of the reaction.

How can such standard curve be constructed?

Bruce Neagle

Typically, the spectrophotometer is 'zeroed' using a sample blank (everything except the enzyme) that has an absorption at the wavelength of interest.

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