I'm doing morphometric analysis of splenocytes and from the data that I have processed up to now I see quite strange numbers for Feret diameter and I wanna know how big are splenocytes?
Some publications indicate size that range from 8-12 um (peripheral blood Ly) while altered cells (enlarged) above 12 um. How ever other textbook data are that the size of Ly - normal - is fro 8-25 um! This "subtle" differences are the point of my work/analysis can you help me with some suggestions/references?
Neutrophils have 12-15µm in blood smears and 8-9µm in suspension.
Naive Small lymphocytes have 7-10µm and large activated lymphocytes have 14-20µm.
The size of the monocytes can vary considerably ranging from 10 to 30µm.
Immature DC have 6-9µm and mature activated DC have 10-15µm.
But the nucleus morphology is more important and elusive than the cell size.
I have culture of spleen cells (where the most of the cells are Ly), what do you mean by the statement activated Ly? Cells that are dividing?
Large Activated Lymphocytes are lymphocytes that were activated by dendritic cells loaded with cognate antigen and became a lymphoblast.
I found in literature the data that point to blast transformation under the influence of specific and non-specific chemicals and since we are testing the effect of some chemical I was leaning in that direction, here is picture of the cells with treatment. Here is the picture of Gimsa staining under immersion objective lens. What do you think?
I couldn't see any cells in this picture. Maybe you can change the light, filter or focus
Dear Ibrahim, thank you very much for your answer! My cells are resting and I'm evaluating the effect of compounds on those cells. From the picture i posted in my answer it seams to me that these cell's are transitional/blast like? What do you think? Do you have any reference (book or article) for the cell size you have mentioned that i could cite?
Consult any textbook of hematology or clinical chemistry and ask somebody who can guide you personally through the anatomy of granulocytes,lymphocytes and monocytes. You can not feel SAVE without personal feedback from an experienced person.
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