No title

More than 33 million infections with more than one million deaths worldwide as of 29 Sept 2020, the Wuhan pandemic is a around 10 months long nightmare and showed no signs of improvements. The...

28 September 2020 9,840 6 View

Role of Ivermectin in managing COVID-19 pandemic...?

03 April 2020 4,784 11 View

Use of Large Waveguide Port Extension Coefficients?

I have tried to design a third iterated Sierpinski Gasket fractal antenna of a standard 88.9 mm height for multiband operation. This antenna resonates at 0.52 GHz, 1.752 GHz, 3.712 GHz and...

28 December 2019 6,168 2 View

Can anyone tell me how to iterate raster in model builder in Arc GIS 10.2.2 version?

i need to subset some set of raster datasets (around 300 files). i read about the model builder. with the help of iterator this can be done. i have implemented the model it gives an error while...

21 February 2018 1,832 5 View

Like Trees of Delhi which gives easy keys for tree identification in Delhi is there any book of similar kind for Uttarakhand State flora ?

15 April 2017 5,515 8 View

How to solve Partial differential equations by using finite element method?

I am trying to solve set of PDE's by using Gelarkin Finite element method for gaseous phase adsorption of VOCs on activated carbon for drawing breakthrough curves. I have used a quadratic...

03 January 2017 5,777 4 View

Forest area of India?

may i know from where i can get the shape file of forest area for India or for particular state?

24 October 2016 6,932 3 View

Has anyone run dsRNA immunoblots?

Hi everyone I am trying to run an immunoblot for dsRNA molecules of specific lengths. I have been running 5% PAGE gels and transferring the RNA using semi-dry transfer @ less than 10V. Note: the...

22 August 2016 3,101 1 View

Ways around expressing a protein which is seemingly toxic for the cells at higher concentrations in mammalian cancer cell line?

Hi everyone Background: Expressing a membrane protein in A549 cells (lung epithelial cancer cell line) using a expression vector under the constitutive CMV promoter. I am able to detect the...

29 March 2016 1,027 3 View

How to express a fusion protein where one of the protein is a receptor protein?

28 January 2016 6,864 4 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View