Hello everybody
TPC assay involve the use of Folin Ciocalteu and sodium carbonate at different concentrations, but how are they standardized? For example, hydrochloric acid is standardized through titration with sodium hydroxide to confirm its concentration (%, N or M) is well prepared. Personally, I think it is important because standardization assures correct preparation of reagents and allows the researcher the successful assay.
Thank you in advance
Dear Sir. Concerning your issue about the preparation and standardization of total phenolic compounds (TPC). This study presents a review of the Folin–Ciocalteu (F–C) assay for total phenolic content (TPC) determinations and describes different approaches to improve its specificity. Phenolics are regarded as the molecules with the highest potential to neutralize free radicals. Therefore, their quantification is a common practice in different areas of food research. However, when determining TPC in plant food extracts, the presence of reducing interferants [ascorbic acid (AA)] produces inaccurate estimations of TPC values. Different methodologies have been proposed to improve the specificity of the F–C assay. These methodologies include: (i) the use of solid phase extraction (SPE) cartridges to separate interferants from phenolics; (ii) the calculation of a corrected TPC value based on the AA reducing activity present in the extract; and (iii) the pre-treatment of extracts with oxidative agents prior to TPC quantification. These methods are described in detail in the present study. Likewise, their advantages and disadvantages are discussed based on new experimental data. A simple modification of the F–C assay procedure is proposed to quantify both the TPC value and the AA reducing activity in plant food extracts. Values obtained by the modified F–C assay can be used to estimate a corrected TPC value. I think the following below links may help you in your analysis:
http://pubs.rsc.org/-/content/articlelanding/2013/ay/c3ay41125g/unauth#!divAbstract
http://www.ijpsr.info/docs/IJPSR14-05-12-014.pdf
Thanks
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