Apart from the size difference, what are the differences like visual apperance, properties, etc.
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Is it possible to tell Proturan families apart based on overall appearance? All the keys I have seen are based on very fine characteristics only readily visible on a slide. Also, there seem to be...
14 June 2024 6,334 0 View
Hello :) I am looking at the presence of toxin biosythensis genes in lake sediments. Nothing fancy, since I only want to verify the specificity of PCR results for primers related to marker genes...
27 May 2024 392 4 View
I wish to resolve IL-6 (mol wt= 21-26KDa), TNF- alpha (17.3-17.4 KDa), GMF-beta (16.7 KDa), GAPDH (36kDa) and PDL1 (40-70 KDa). I think 12.5% gel is good to go. Is it advisable or should I go for...
25 April 2024 9,798 0 View
Hello, Since a large part of my budget will go on NGS and I don't have the money to repeat it, I want to make sure that it will work. The PCR products look good on the gel (nice bands of the...
21 April 2024 7,785 3 View
I am running ELISA for different biomarkers against meningitis. I need to know how to calculate cutoff values for it in ELISA. There is no negative control or positive control for these kits,...
07 April 2024 3,079 1 View
So I know we should round the value of R Square to two decimal points, but how should you do it if the value is .001. Should you report it as R2 = .00, or R2 = .01?
26 March 2024 1,387 2 View
Has anyone run slide in Visium of tissue sections (Heart, kidney, brain) with CMC than OCT for spatial transcriptomics ???
14 March 2024 243 2 View
I have selected two deep learning models CNN and sae for data analysis of a 1 d digitized data set. I need to justify choice of these two dl models in comparison to other dl and standard ml...
18 January 2024 6,800 1 View
I have used two dl models CNN and sae for data analysis of 1 d digitized data set. I need to justify use of these two models in comparison to other dl and other models. I am using ga to optimize...
18 January 2024 9,816 0 View
I have three nanogels.. The difference between them is the cationic monomer ratio... However, the test is giving me similar curve of the three formulations
27 May 2024 2,064 0 View
I have made a natural product loaded nanogels. Through MTT assay I have found out the drug dose but am not getting how to treat the cell further with the formulations
04 March 2024 5,389 1 View
I have synthesised a 100 nm sized cationic nanogel and I want to use it as a carrier for cisplatin and paclitaxel
03 March 2024 3,426 0 View
I also wonder if drying with a freeze dryer or oven have any effect on the amount of swelling ratio.
10 November 2023 336 3 View
I prepared chitosan nanogels with chitosan and TPP by ionic gelation method. I concentrated nanogels by ultrafiltration centrifugation. The result concentrated solutions (pH 5) was diluted with...
25 October 2023 6,780 0 View
I have been following the paper https://doi.org/10.1039/C7NR06732A to synthesize them, but I could not synthesize microgels with a Polydispersity Index of less than 0.01. My microgels have a...
26 April 2023 2,216 1 View
I am trying to covalently attach the RGD peptide sequence to PNIPAM-co-AAc microgels already coated on different substrates (Si, Glass and Polyurethane). I am going to use the common carboxylic...
10 April 2023 8,034 0 View
Dear beloved scientist. I have a question regarding Polyvinilcaprolactam Nanogels (PVCL NGs) synthesis. I used 500 mg VCL, 20 mg BAC, 10mg of SDS and 12.5mg of NaHCO3 were dissolved in 100g of...
02 March 2023 9,178 0 View
I am an M.pharm student working on project regarding keratin alginate nanogels where i have to maintain nitrogen atmosphere during the formulation process. Can anybody tell me how do i obtain and...
28 November 2022 5,161 4 View
when i use cationic monomers and temperature-sensitive monomers to synthesize nanogels by precipitation polymerization , how to modulation of nanoparticle surface charge?
25 October 2022 3,014 3 View