@ Ibrahim, Collagen nanoparticles can be prepared through three main techniques: chemical, physical, and self-assembly methods. Chemical methods consist of coacervation or complexation and emulsification. Nano collagen can also be fabricated through milling. Milling is a process whereby mechanical energy is applied onto a polymeric material to be broken down into fine nanoparticles. Milling is an inexpensive way to reduce particle size on large production scales. The attached file may show you how you may prepare collagen nanoparticles by self assembly methods.

Ibrahim Alghoraibi

Collagen is obtained from bones in the technological process of obtaining gelatin. The bones are crushed, treated with lime (the process of gilding), washed, treated with an aqueous solution of hydrochloric acid (maceration). Get collagen.

Ibrahim Alghoraibi

Hi

Polymeric nanoparticles can also be prepared by electrospraying technique. The principle of electro-spraying is to apply a high voltage to a polymeric solution to force the polymer to come out from the syringe in the form of nanoparticles. Electrospraying has emerged as a technique similar to electrospinning, which uses analogous technology to produce nanostructures.