How and Why NH4+ and Mg2+ have antagonistic effects during PCR stabilization?

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Paul Rutland

I am not aware that these 2 reagents are antagonistic but they do serve different functions. NH4 alleviates the problem of primer to template mismatches by destabilising weak hydrogen bonds that form during a mismatch but magnesium is both a divalent ion so has a large effect on the annealing temperature of primer annealing and is also essential as a cofactor for polymerases so is essential in pcr. Using both reagents together makes the pcr more stable with respect to both annealing temperature and magnesium concentrations

Alomgir Hossain

Follow this article and gain better idea...

Article Antagonistic effects Na+ and Mg2+ on the structure, function...

Walid Khalilia

Dear Shubham Koirala I agree with Paul Rutland