Qiime2- No reads passed the filter. How to set the trunc value?

Hi, I am working on some 16S sequencing data, and seems some of them are low quality. I am not sure how to set the trunc value in dada2. qiime dada2 denoise-paired \ --i-demultiplexed-seqs...

30 January 2021 6,213 3 View

What is the best way to prepare anaerobic medium?

Hi, I would like to prepare anaerobic medium for cultivation of gut microflora. I have an anaerobic chamber. How should I remove oxygen from the medium? Is it enough to leave it for 48 hours in...

05 October 2020 3,093 4 View

Any online tools for metagenomics data analysis, specifically 18S rRNA?

Could you please recommend any online tools for metagenomics data analysis for 18S rRNA?

01 March 2020 5,424 5 View

Is it possible to do SEM on a cross-section cut of a fresh leaf?

Hello, I'm working with pulsed electric field applied to lemongrass leaves to generate pores on the cell membrane to enhance essential oil extraction. I would like to know if there is a way to...

20 October 2019 1,364 3 View

How can I preserve leaf samples to measure the trichome density?

Hi. I'm working in a tropical rainforest and I need to measure the density of trichomes, but due the lack of equipment in the station, I need to preserve the samples during 1 month to then count...

10 October 2019 1,460 4 View

Intracellular staining of peritoneal mast cells for cytokine detection by flow cytometry?

Hi! Can anyone suggest a protocol for intracellular staining of peritoneal mast cells for cytokine detection by flow cytometry?

19 March 2019 9,262 3 View

Any experience/opinion on sociolinguistic interviews without the interviewer, i.e., only a questionnaire and the interviewees recording themselves?

I want to interview non-native speakers of English, but I am not a native speaker, so to avoid certain implications of this (unnatural setting, accommodation, etc.), I am thinking of sending them...

11 October 2017 4,867 4 View

Does anyone know of a good reference that describes the biology of monocyte/macrophage recruitment and differentiation during wound healing?

Please send me the title/author if you know of a good reference. Thank you!

04 October 2017 7,695 3 View

How do I do immunocytochemistry staining on non-adherent cells?

Are centrifugations required between every wash step/blocking/antibody incubation in order to not lose the cells? Is there a way to make them adhere through a fixative or some other process in...

03 October 2017 8,611 4 View

Staining plant by Safranine O Fast Green FCF?

14 March 2017 1,404 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

How to analyse monosaccharide compositions from soluble fiber?

I have tried to hydrolyze the sample using 2 M TFA at 100C for 24, 48 h and 120C for 2 h. From these conditions, the HPLC results showed that peaks were not separated into monosaccharide. I found...

17 February 2021 5,230 1 View

No title

Hello. I ask for help - I need to get lithium tetraborate (soluble salt) from lithium carbonate and boric acid. In literature, everything seems to be simple - mix, ignite and - it's done. But it...

16 February 2021 9,523 2 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

Which non-coordinating solvent dissolves anhydrous Iron(II)chloride?

Hallo, i am looking for a non coordinating solvent that dissolves anhydrous FeCl2 for a synthesis in which i can not use solvents like thf, pyridin etc.. I already tried benzene (no solubility),...

11 February 2021 6,105 1 View