06 May 2022 0 9K Report

Could someone recommend how to prepare and store proline stock solution to be used as a medium supplement?

I need 40 mg/ml final concentration in medium. Solubility in water is 1,62 g/ml at 25 C.

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How can I avoid uneven IHC staining on free floating tissue from frozen human CNS sections (cryostat 50um thickness)?

Hello, I've been doing free floating IHC using a VAChT antibody with a 1:1k dilution (139 103 - Synaptic Systems). However, there has been a struggle to keep an even stain with my samples of...

06 July 2024 4,521 2 View

Problem with YES estrogen bioassay. Estrogen contamination?

Hello everyone, I've been encountering a recurring problem with the YES (Yeast Estrogen Screen) test, specifically related to the development of an intense red color after adding CPRG. I'm using...

07 February 2024 1,144 0 View

Is there a way to separate plasma from lyzed red blood cells when whole blood samples have been frozen at -80°c?

I am currently engaged in the analysis of a collection of previously obtained samples, which primarily comprise frozen whole blood. My specific requirement pertains to isolating the plasma...

08 January 2024 1,842 1 View

Anyone have any fairly recent info/research on human and non-human primate bonding?

At the moment I am interested in human and non human primate bonding, especially in captive management, but more widly too. The causes and consequences, pros and cons, how it could inform our...

17 December 2023 9,236 0 View

Problem with Trizol RNA extraction - what should be purity of chloroform and isopropanol?

Hi, I'm trying to isolate RNA from cell cultures using the Trizol method. However, all my samples have low 260/280 values (~1.6). Could this be because I used chloroform and isopropanol, which was...

26 October 2023 9,909 6 View

How can I know the number of particles per vial of the pH rhodo s aureus bioparticle reagent?

Hello, I am working with the aforementioned reagent, but my problem arises that the reagent does not indicate the quantity of particles that come per vial, that is, the number of particles, it...

11 October 2023 817 1 View

Can someone recommend a good CRISPR identifier?

Hi, I am a beginner in bioinformatics and I would like to identify CRISPRs in my MAGs fasta files. Can someone recommend an up-to-date good tool that can be easily installed through the Conda...

21 May 2023 3,549 3 View

How calculate heat gain in PVT modules?

how to calculate heat gain from solar cells in pvt modules

27 April 2023 6,289 1 View

Do warnings of non-convergence in a trait.glm analysis always indicate that the model is not valid?

I'm working with a trait.glm analysis from the mvabund package in R. I have two datasets that look exactly the same with the same amount of environmental variables and the same functional traits,...

15 February 2023 6,547 3 View

Does anyone know how to run an latent moderated mediation in mplus?

The problem is that model indirect is not available for type is random option. Thanks

14 February 2023 1,269 1 View

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