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Questions related from Paulina Anna Trzaskowska
Hello, I am going to indicate total glycosaminoglycans content as well as DNA content in chondrocytes culture (48 well plate). First I have to prepare papain lysates out of the cells and according...
16 January 2023 6,300 0 View
Hello, Could you please recommend DAPI concentration for nuclei staining in mesenchymal stem cells during chondrogenic differentiation? I have used 1 μg/ml DAPI for 6 min, then as usual PBS...
08 December 2022 9,256 3 View
I would like to perform real time study on the effect on various differentiation conditions on the collagens production in MSCs during differentiation into chondrocytes. I would like to visualize...
15 November 2022 7,377 2 View
Could someone recommend how to prepare and store proline stock solution to be used as a medium supplement? I need 40 mg/ml final concentration in medium. Solubility in water is 1,62 g/ml at 25 C.
06 May 2022 8,683 0 View
Dear all, Do you have the experience in MSC chondrogenic differentiation and immunostaining at the specified time points? I used to work with adherent cells only so I feel a little uncertain. As I...
01 January 1970 2,105 9 View
