Paulina Anna Trzaskowska

5 Questions 11 Answers 0 Followers

Questions related from Paulina Anna Trzaskowska

Blyscan kit - Glycosaminoglycans content - freezing?

Hello, I am going to indicate total glycosaminoglycans content as well as DNA content in chondrocytes culture (48 well plate). First I have to prepare papain lysates out of the cells and according...

16 January 2023 6,305 0 View

DAPI concentration for mesenchymal cells staining during chondrogenic differentiation?

Hello, Could you please recommend DAPI concentration for nuclei staining in mesenchymal stem cells during chondrogenic differentiation? I have used 1 μg/ml DAPI for 6 min, then as usual PBS...

08 December 2022 9,267 3 View

Does anyone know if there are any possibilities to vital stain collagens in mesenchymal stem cells?

I would like to perform real time study on the effect on various differentiation conditions on the collagens production in MSCs during differentiation into chondrocytes. I would like to visualize...

15 November 2022 7,380 2 View

Ho to prepare proline stock solution for cell culture?

Could someone recommend how to prepare and store proline stock solution to be used as a medium supplement? I need 40 mg/ml final concentration in medium. Solubility in water is 1,62 g/ml at 25 C.

06 May 2022 8,687 0 View

Mesenchymal stem cells: chondrogenic differentiation and immunostaining & SEM fixation

Dear all, Do you have the experience in MSC chondrogenic differentiation and immunostaining at the specified time points? I used to work with adherent cells only so I feel a little uncertain. As I...

01 January 1970 2,109 9 View