Dear fellow researcher,
To calibrate real-time PCR assays for the detection of several viral species, we are using the NIBSC standards.
I am writing to ask if you have any experience working with them, specifically with HIV, HBV & HCV (NIBSC code: 12/224, 10/266 & 18/184, respectively).
Mainly I am clueless regarding where it says:
"Once reconstituted, the International Standard should be diluted in the matrix appropriate to the assay e.g. in HCV RNA-negative plasma, and should be extracted prior to HCV RNA measurement."
I am thinking if the receivers of these standards need to perform an extraction step before being able to use them for test calibration purposes, due to the numerous variables that such a step would add, will the resultant material still have the features to be regarded as a "standard" sample?
I have attached HIV, HBV & HCV's instructions for use files for your reference.
Eagerly looking forward to your views.
Best wishes,
Negar
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