Histopaque 1083/1119 in rat neutrophil islotion?

06 September 2016 1 3K Report

Some articles say that use Histopaque 1083 and 1119 can islotion neutrophils from rat peripheral blood ，but i can not see a clear neutrophil layer anymore ?can someone give me a method for it ,please.

Goodwin G. Jinesh

Just use Sigma granulocyte protocol for histopaque. But it seems the starting material is less (because rat blood).

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