Cannabidiol powder solubility and stability?

Hello, I'm interested in knowing if cannabidiol is stable for long term storage in methanol or DMSO in recommended solubility mix for biological studies and in which conditions. Thanks.

21 October 2019 2,445 1 View

Any recommendation for wheat germ agglutinin kit\protocol for rat's cardiac tissue- formalin-fixed and paraffin-embedded?

I plan to stain atria+ventricles tissues.

11 May 2018 5,536 3 View

Which resources could help residents develop a successful grassroots participatory process ?

In my article "When knowledge is power: grassroots participatory initiative as a process of resource development" I claim that residents are initiating informal grassroots participatory...

25 December 2017 1,657 12 View

How long can I keep atria/ventricle section's in Formaldehyde after the sacrifice until starting the Fixation and Dehydration process?

21 January 2017 6,987 2 View

Does anyone have experience working with GP120 and SF9 cell transfection?

I am currently trying to perform a transfection of GP120 baculovirus into SF9 cells. However, my western blot keeps showing large numbers of nonspecific bands that show up even in the negative...

02 December 2015 5,332 1 View

Can I select for IPTG-induced fluorescent e. coli colonies on LB-Agar?

I am doing recombineering and would like to swap out GFP for mCherry in my vector. I can proceed with the GalK selection scheme but I tried it once and didn't work so I am trying to think of...

05 October 2014 1,713 3 View

How do we define influencers in influencer marketing?

Hi all, so much has been said and written about the role of influencers in influencer marketing. So far, most studies focus on influencers in terms of their number of followers, thus we have nano,...

01 January 1970 6,419 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View