Is there a way to calculate DM without using external pectin standards of known DM? Thank you very much.
You have to calibrate i.e. run series of pectin samples with varied DM... there are two distinctive bands around 1700 cm-1, but you can not just integrate them, there is spectral interference from other compounds. There has been many publications on this subject in Carb Polymers in the 1990's early 2000..
Hi Catherine,
Thank you for the answer. Really appreciate it.
Hi,
Just wondering, is it possible to apply this equation:
DE (%) = [A (1740) / A (1740 + 1630) ] x 100
where A is either peak "absorbance intensity" or "area", without using the series of pectin with known DE?
Thank you.
No, there is definitely interference from other compounds including a water signal, you'd have to deconvolute the spectrum Carb polymers 42 (2000) 359-368.
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