We have grown some plant endophytic bacteria in TSB medium. Now we are trying to grow them in liquid medium (TSA) for genomic DNA isolation ,some are growing and some are not ,what can be the reason?
Isolation of bacteria is accomplished by growing ("culturing") them on the surface of solid nutrient media. Such a medium normally consists of a mixture of protein digests (peptone, tryptone) and inorganic salts, hardened by the addition of 1.5% agar.TSA is used for cultivation of non-fastidious bacteria.Tryptone Soya Broth (TSB) with agar are growth media for the culturing of bacteria. You can supplement the medium with blood to facilitate the growth of more fastidious bacteria or antimicrobial agents to permit the selection of various microbial groups from pure microbiota. As with any media, minor changes may be made to suit specific circumstances. TSA is frequently the base media of other agar plate type, i.e. blood agar plates (BAP) are made by enriching TSA plates with blood.
Thank you Sir...but I am not getting why some bacteria is growing in TSA but not in TSB .where medium composition is same only difference is agar.
Hi.
Try to check the REDOX potential in the TSB . How much
sediment is being recognized? Did you incubate with shaking
or not?
Hello.
Might be a stupid question but: did you vortex the broth before incubating?
It's important to vortex the broth culture before the incubation if the incubating period is with no shaking.
Thank you all for your valuable comments....I kept it in shaking incubator for growing 37 C...The thing I am finding now these bacteria are not growing in Liquid or solid media (TSB or TSA) ...what is the reason ..are bacteria died?
It is a possibility that your bacteria is using agar to grow. It generally happens in mixed cultures of unknown bacteria.
Hi Badrul, i also found the same phenomenon. My bacteria can grow on R2A agar, but not in R2A broth. I also don't know why. but i think it was related to quorum sensing. The bacteria might need some denstity to grow up.
In our lab we focus on research of bacteria from habitats polluted by organic pollutants, heavy metals, etc. and we encounter such a phenomenon very often. In one particular case, amendment of e.g. sterilized sea sand showed up to support the growth of such problematic bacterium (apparently, it requires some kind of 'solid support' for growth).
Ammendment: I agree with @Parkash Rawat - for instance, the majority of environmental Pseudomas spp. grown in our lab can utilize agar used for solidifiying media, without any additional carbon source. Not even the use of Noble agar (i.e. purified) represses this unwanted growth, it just slows it down.
I have thé same problem, my bacteria does not grow on liquid or solid medium (any medium) . Are bacteria died ?
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