Hi, I'm looking for a small amount HER4 IHC antibody (ERBB4) for about 40 samples But it is not available in Bulgaria. Can you help me?

More Julian Ananiev's questions See All

Bell's inequality from probability theory?

It is said Bell's inequality is a consequence of probability theory, which has nothing to do with quantum or not quantum. There are many papers discuss this issue, but I don't know which one is...

22 December 2020 8,254 3 View

What are the latest (theoretical) understanding of self-interference (in double-slit exp)?

In 1967 R. L. Pfleegor and L. Mandel, Phys. Lett. 24A, 766-67 proved experimentally the self-interaction of photon through double slit. Well, some said it is even later until 1986 by Grangier et...

19 November 2020 2,378 11 View

Can Copper be sputtered (via PVD) onto Platinum-Iridium to improve solderability?

Hi! First time poster on RG! I would like to solder (ball grid array reflow) onto a Platinum/Iridium alloy without the use of special Platinum soldering fluxes. Can copper be sputtered onto...

18 November 2020 2,105 3 View

Which tool for operon prediction in bacterial genomes ?

Hi, I want to predict operons on entire bacterial genomes (already annotated). I used to use operon-mapper ( https://academic.oup.com/bioinformatics/article/34/23/4118/5040321), which is great but...

07 October 2020 7,192 7 View

Can I merge z-standardized items of a 5-point and a 7-point Likert scale into one scale? (psychology)?

I am investigating a new topic with an established questionnaire. However some items are not suitable so the scales are not the best predictors in my hierarchical regression (n.s.). However, some...

04 October 2020 4,630 6 View

Optimization with Gaussian using a Cluster?

Hi all, I just try to perform a calculation for a cluster of TinOxide for determining a reaction mechanism in an organic reaction. I tried to run this calculation using a mixing basis set...

13 May 2020 8,642 4 View

Problem with Gasussian ?

Hi dear all, I just carrying out a computational run of a single point energy at MP2/6-31g++(2p,2d)//B3LYP/6-31g++(d,p), but when I tried to do the refinement at MP2/6-31g++(2p,2d) the next error...

27 March 2020 5,759 3 View

Human Cell lines with SNP genotype information?

I want to design BaseScope probes for human samples of a certain gene in his Wild-type and his mutated form (single nucleotide point mutation). To do that I need to provide stable cell lines that...

14 March 2020 3,063 2 View

Does anyone know how to do a moving heat source in cylindrical coordinates for a pipe welding simulation in ANSYS apdl?

I am doing a pipe welding simulation using the goldak model in ANSYS apdl. For doing this I would like know how can I simulate a moving heat source in cylindrical coordanates and how to implement...

05 March 2020 8,673 4 View

How can the x-ray spectrum of a spinel structure be interpreted to know Miller indices?

I have to interprete a kind of LTO samples with structure spinel in X-ray diffraction analysis but I don't know how I can get Miller indices. Thank you

11 February 2020 6,110 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View