Hi, I'm looking for a small amount HER4 IHC antibody (ERBB4) for about 40 samples But it is not available in Bulgaria. Can you help me?

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Are there open databases for mortality or large cardiovascular events such as Framingham?

Are there open databases for mortality or large cardiovascular events such as Framingham? To be able to train predictive models of cardiovascular events with a large number of patients.

08 July 2024 6,133 2 View

¿ Los paises en vias de desarrollo son mas abiertos frente a las politicas migratorias?

Es posible observar que mientras que paises como colombia con altos indices de pobreza y desigualdad, abrieron sus fronteras a millones de migrantes venezolanos, por otro lado algunos paises de la...

16 June 2024 236 2 View

How many populations in the world have been repopulated thanks to migration?

Existen diversos conflictos y problematicas sociales en el mundo que han causado migraciones. Por ejemplo, la migración venezolana y la reciente migración debido a la guerra entre ucrania y rusia....

16 June 2024 6,989 1 View

MDR1 (ABCB1) western blotting?

Has anyone seen MDR1 bands below 75 kDa? I am using a monoclonal antibody and I am expecting a band at 170 kDa

15 May 2024 8,690 4 View

How can I dissolve my drug in 0.5% methylcellulose?

I am preparing a drug for oral gavage which needs to be dissolved in 0.5% methylcellulose. The drug powder remains as clump and refuses to go into solution. Any tips on this?

01 April 2024 4,859 3 View

Is there a need of web security practices even when the students are fully aware?

I'm a student researcher that is studying everything about the student's awareness on web browser and online behavior and safety practices. I wanted to know if there's a need of handout/mini book...

13 January 2024 8,315 5 View

QSWAT error - failed to run the model after step 3 in the simulation process. Please share possible solution?

Hello friends, I'm trying to run QSWAT and I'm after step 3 on the simulation process , setup my model from 2001 to 2005 with NYSKIP 2. Chose daily because my data prepared like that. Before that...

11 January 2024 6,186 1 View

How to do lentiviral transduction with two different vectors?

If I want to transduce cells with two lentiviral vectors, do I perform the transduction one after the other? Or do I collect the lentivirus from the different HEK plates and co-transduce my target...

07 November 2023 7,632 5 View

How to lyse bacteria cells gently?

Dear community, I’m trying to lyse Lysobacter enzymogenes cells. I want to open them up in a very gentle way to cause as little damage to everything that’s inside them (proteins, peptides,...

08 October 2023 2,045 0 View

Could EtCO2 be higher than PaCO2 in a congenic heart diseased child?

Could be any scenario that you could find a higher EtCO2 than the value of a PCO2 of a ABG in a children with a congenic heart disease under invasive mechanical ventilation? In my case, a patient...

20 September 2023 7,746 1 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

Questions of antibodies selection?

Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...

15 July 2024 6,008 5 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

How to measure the concentration of monoclonal antibodieswithout nanodrop ?

Dear all, I'm going to radiolabel antibodies. So first of all, I'll remove excipients using the centrifugation method with amicon. Once I'm done, how do I calculate/measure the concentration of...

14 July 2024 3,106 3 View