If I want to transduce cells with two lentiviral vectors, do I perform the transduction one after the other? Or do I collect the lentivirus from the different HEK plates and co-transduce my target cells?
Depends on what selective markers you have.
One has Blasticidin and the other Neomycin
As you can use both selective markers at once just try a double transduction.
Thanks Robert
Isn't it better to perform a transduction with a polycistronic vector? Or do you want to test different MOIs?
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