OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Quality of antibodies from Creative Biolabs?

It seems Creative Biolabs has everything and so many versions of antibodies not owned or sold by any other company. How reliable are their products? I am looking for a clone where potentially...

08 February 2021 2,167 3 View

Benefit of CTCF (corrected total cell fluorescence)?

03 February 2021 6,302 2 View

Why do the absorbance values of different concentrations of ascorbic acid solutions mixed with DPPH free radicals stay constant?

26 January 2021 458 14 View

Interpreting regression findings prior to inclusion of control variable?

I'm looking for advice on interpreting a statistical relationship - it's for a paper I am reviewing. (Variable names have been changed). It was found that: [IV anxiety + control variables y and...

06 January 2021 558 3 View

Can someone help me with an extraction protocol?

Hi all, Does anyone have a protocol for extracting DNA from agarose that is only 120 bp without a kit? The protocols I have tried are for much larger fragments and it is just not working. Thank you!

10 September 2020 1,291 2 View

Advice for getting dermatopathology tissue to stick to slides during immunostaining?

I work in a veterinary pathology lab and we do immunohistochemistry staining by hand on all types of tissues that are FFPE. We have particular difficulty getting dermatopathology (skin) samples to...

26 August 2020 792 1 View

Validation of experimental results?

Is it neccessary to perfrom validation of process model established using a box-benkhen experimental design? How "big mistake" will it be if I finish the experiment after performing the runs...

24 August 2020 6,917 3 View

Any reliable protocols for tissue detection of DNA damage with IHC or IF?

Dear all, Hope as a community your are doing fine. I have a question regarding antibodies against gH2AX, XRCC1 and p53bp1 that have worked for tissue detection for either IHC or IF, using...

08 June 2020 1,461 3 View

Where can I find the parameters of Miltenyi gentleMACS protocols?

I'm planning to use a Miltenyi brain tumour dissociation kit, but without the gentleMACS machine. Does anyone know where I can find the parameters of the machine's programs/protocols?...

16 December 2019 6,150 1 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Which regression is best fit when moderator is categorical and dependent variable is latent in nature?

I am using categorical variables as a moderator variable, and my dependent, mediator and IV variables are latent in nature. Which regression test I can apply. As logistic regression is for binary...

11 February 2021 1,584 3 View

Professional editing and proofreading services?

08 February 2021 7,901 6 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Can anyone help me in this MATLAB Coding?

Hello, I have a dataset excel CSV sheet with a three columns,(X,Y, and Z) The first and second columns represents the coordinates of point, while the third one represents the elevations value of...

04 February 2021 3,451 3 View