Hi, i want to check the stability of my designed protein in which i've replaced a 5residue beta/alpha loop can any one tell me the process or any tool...

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Rajashri N Bhairamadgi

hi...

have look on this info may be useful

1) iPTREE-STAB-

it is an interpretable decision tree based method for predicting protein stability changes upon mutations.

http://210.60.98.19/IPTREEr/iptree.htm

http://210.60.98.19/IPTREEr/input.jsp

2) CUPSAT

It is used for prediction of protein stability upon point mutations

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1538884/?tool=pubmed

http://cupsat.tu-bs.de/

thank you

also try 3D-PSSM

Raja shekar Varma Kadumuri

Thnx for the reply i wl look at it..........n wish u happy new year in advance....

Kapil Choudhary

u cn try arguslab or PyRx for manipulations and HEX server online for virtual screening. all are freewares.

Happy new year you too....

Cindy Castado

Other tools for stability prediction after mutations :

- Concoord/PBSa : http://ccpbsa.biologie.uni-erlangen.de/ccpbsa/

- PoPMuSic (but I don't know if it works for a complete loop) : http://babylone.ulb.ac.be/popmusic/

thanks cindy, Concoord/PBSa is so cool thank u very much.........