I want to check stability and activity of my designed protein can any one help me by giving info regarding what are the best softwares that are available
You can try the following tools for Thermal Stability prediction for mutants:
Imutant2.0
Popmusic
Cupsat
FoldX
For a recent review of progress in this area and a more comprehensive list of methods, see the following paper:
PLS FOR PREDICTION OF THERMAL STABILITY OF PROTEIN MUTANTS
S Talluri, Journal of Advanced Bioinformatics Applications and Research 2 (2), 155-160, 2011
http://bipublication.com/files/JABARv2i2201106.pdf
Thank for ur reply it help me a lot........
Although the answer replied is late but I think it would add to knowledge for others too.
prethurmut: http://www.mobioinfor.cn/prethermut/index.htm
automute : http://proteins.gmu.edu/automute/AUTO-MUTE.html
PROMPT: http://promtp.bsi.tw/
Tm predictor: http://tm.life.nthu.edu.tw/
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