Raja shekar Varma Kadumuri

9 Questions 14 Answers 0 Followers

Questions related from Raja shekar Varma Kadumuri

Suitable expression system for 30-40 residue peptide His tag or GST ?

Hi, I would like to express and purify a peptide of 30-40 residue long. I would like to know if I can use pET-31b vector with HIS tag or should I go with pGS-21a with GST tag. It would be helpful...

12 December 2014 926 5 View

Is it possible to correlate stability difference between different peptides by doing unfolding MD simulation at 400K?

09 September 2013 4,610 7 View

How to upload multiple pdb files in jmol applet, and execute scripts on required pdb file ?

Is there any method to upload multiple pdb files in jmol applet, and execute scripts on required pdb files..

08 August 2012 503 1 View

Is there any script or software available to calculate distance between End C-alpha (C-alpha end to end distance) in a peptide sequence?

I would like to calculate distance between end c-alpha atoms in some peptide sequences, I'm trying to accomplish this task by writing some perl scripts but i'm facing some errors in my script so...

08 August 2012 8,875 6 View

Can a protein model, i.e. designed using abintio method, be used as a template for the same sequence in modeller to obtain better refined structure?

I've gone through some papers where the similar method was used i.e. a structure defined by abintio was used as a template for the same sequence to obtain better refined structure. I would like to...

05 May 2012 2,830 2 View

I want to super impose my loop structures based on the topology ?

I want to super impose my loop structures based on the topology, so i would like to download cora suite,but i am not able to find it anywhere pls help me if any one has info regarding cora suite...

02 February 2012 9,396 1 View

Can any one tell me how to get PROFASI software,and also is there any other alternate softwares to it.............

01 January 2012 1,364 0 View

Hi, can any one tell me what are the best softwares (online/downloadable) for checking stability and activity of a designed protein.

I want to check stability and activity of my designed protein can any one help me by giving info regarding what are the best softwares that are available

12 December 2011 4,039 3 View

Hi, i want to check the stability of my designed protein in which i've replaced a 5residue beta/alpha loop can any one tell me the process or any tool...

Hi, i want to check the stability of my designed protein in which i've replaced a 5residue beta/alpha loop can any one tell me the process or any tools available.........

12 December 2011 8,516 7 View