Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View

How to determine the genetic potential of the genotype in terms of fruit size and weight?

In addition to genetic and environmental factors, fruit size is also influenced by crop load, rootstock, orchard planting system/tree training system, pollination, water and nutrient management,...

13 January 2021 9,309 4 View

What are technical problems working with HCT116 colon cancer cell line ? how is it maintained and cultured to study the anticancer effects of a drug?

Technical problems working with HCT116 compared with other cell line Caco-2 and HT29

07 January 2021 2,067 3 View

Is it possible to ligate two restriction ends those have no complementary bases?

In my lab,we are trying to construct a control plasmid. So we have to remove the insert gene which has two restriction ends:one is XhoI and other one is Hind III.So after double digestion we want...

01 January 2021 3,271 4 View

What are causes of obesity or overweight?

I am writing research orientationed book

24 December 2020 2,595 3 View

Is it machine learning models that have replicability capability?

We are doing the prediction of soil nutrients using spectral information by applying machine learning models. More than 300 soil samples were used from one district for analysis. We got the...

21 December 2020 9,663 3 View

How can I analyse and compare crop yield under different irrigation methods?

I have to compare yield parameters of a tree crop grown under three irrigation methods. But these systems are already established separately in three adjacent fields. Since I can not follow a RCBD...

21 December 2020 3,065 5 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Can I use the Chlorophyll stability index (CSI) to calculate the stress potential of cyanobacteria that are under stress?

I want to calculate the stress potential of cyanobacterial cells that are inoculated with antagonistic bacteria. My results showed that there is a drastic reduction in the chlorophyll content of...

23 January 2021 331 3 View

Why and how to avoid negative values in optical density by spectrophotometry when measurements corrected by blank solvent?

I am trying to assess chlorophyll-a content from coral tissue using spectrophotometry (with SPECTROstar Nano, BMG). However, I always seem to obtain some negative values in my optical density raw...

20 January 2021 1,542 4 View

Why there is need to calculate the chlorophyll content and chlorophyll index. What protocol should I used for calculating chlorphyll in mustard?

I want to know the best and effective method/ protocol to calculate the chlorophyll from mustard plants. Please suggest some relevant and the methods or protocols from which I can easily calculate...

05 January 2021 9,882 3 View

Hello, can i learn amount of Chlorophyll in Spinach as a mg in 100 grams ?

I have looked on the internet and website but i could not find it so i need yours help please.

26 December 2020 8,354 3 View

What is it the relationship between photochemical efficiency and a chlorophyll a fluorescence in a plant ?

I hope somebody could help in explaining this point

10 December 2020 513 4 View

How can I use the selection matrix in Pymol to select atoms by their number within a cofactor?

I work with photochemical reaction centers (like Photosystems I & II) and it can be helpful to use PyMol to create useful pictures of them. With these proteins, the chlorophylls do all the...

04 December 2020 4,778 2 View

Simple clearing method for observation of hand-sectioned wheat leaves?

I am using a toluidine blue O preparation for staining different parts of wheat plants to observe a fungus growing in them. This works really well for lighter tissues of the plant! However, the...

27 October 2020 9,159 1 View

Whether it is compulsory to carry out in-situ water sampling and laboratory-based analysis for a water quality study based on remote sensing?

A study assessed the water quality status using a few parameters such as Chlorophyll a, BOD, COD, TSS and etc to determine the pollution level of water bodies using remote sensing techniques....

19 September 2020 4,839 3 View

Is it possible to carryout Chlorophyllin (Mg-Chl, or Cu-Chl) photosensitized degradation of methylene blue dye in presence of visible light ?

As both Chlorophyll semi-synthetic derivative and methylene blue organic dye has been widely used as a potential photosensitizer in applications like PDT but I want to know that; a chlorophyll...

15 August 2020 6,325 3 View