Y K D D Thathsarani

7 Questions 5 Answers 0 Followers

Questions related from Y K D D Thathsarani

What is the method of preparation of phosphate buffer at pH 4.7 for alkaloid determination ?

I am working on quantifying alkaloids in a plant extract. I want to prepare a phosphate buffer solution with sodium phosphate dibasic dihydrate with citric acid at pH 4.7. However, sodium...

08 December 2023 6,580 3 View

Are there any methods available for quantitative determination of terpenoids, saponins, glycosides and steroids via UV visible spectrophotometry?

Hi all!! I am working on determining phytochemical constituents in a plant extract. I could not find a reliable protocol for quantitative analysis of terpenoids, saponins, glycosides and steroids...

08 September 2023 5,120 0 View

Would it be possible to know how TLC can be performed for plant based proteins and carbohydrates?

Hi all! I am going to work with plant based proteins and carbohydrates. I want to conduct thin layer chromatography experiments for assessing the presence of proteins and carbohydrates (not in...

25 August 2023 5,895 2 View

Is it necessary to freshly prepare the Brewbacker and Kwack medium ?

I am currently working on pollen studies. I have to use Brewbacker and Quack medium as the pollen germination medium. Is it necessary to prepare it freshly? Thank you very much for your help

19 May 2023 5,884 0 View

Hi, What can be used as an alternative for trans cinnamic acid?

Hi I am going to investigate the activity of phenylalanine ammonia-lyase. For the caliban curve I have to use trans cinnamic acid. Are there any alternative for this cinnamic acid since it is hard...

30 March 2023 9,814 0 View

Hi, I had an issue about calculating chlorophyll content?

I used 664nm, 647 nm to measure the absorbance for chlorophylls. I used 10mg of fresh weight dissolved in 5ml of acetone. Could you please help me to find formulae for chlorophyll...

07 November 2022 5,824 5 View

Hi, I am going to investigate the activity of PAL enzyme. I have some issues in the preparation of the reaction mixture.How can I prepare this?

The reaction mixture includes, The extracted enzyme (250 microliter), Tris-HCl (100 mM) Betamercaptoethanol (1 mM) L-Phenylalanine (50mM) The total volume must be 1.5ml The final concentrations of...

03 November 2022 3,643 5 View