Hi, how can I extract stat3 from human spermatozoa and evaluate it by elisa?

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I seed my cell in Opti-MEM?

I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...

07 July 2024 407 0 View

Do you think this contaminations?

Kg1a cell line in 96 well plates do you think this white dots are contaminations or depris ( insides black circle)

29 June 2024 5,154 1 View

Why do my cell lines grow very well in flasks but not in a 96-well plate?

My Kg1a cell lines grow very well in flask, but when I seed it in 96 wells plate give only 25 % viability

24 June 2024 4,596 3 View

Can anyone tell me where are the previous examples code for GANFEThemt devices in Silvaco Atlas? I couldn't find them even in silvaco offical website?

I need to get the previous example codes of silvaco to understand the basic mathematical modelling , meshing and doping of the AlGaN/GaN HEMT

20 May 2024 233 0 View

How crystalline size increase but lattice constant decrease in soft ferrites xrd?

when Mg substituted in Ni Zn ferrites crystalline size increase but lattice constant decrease in soft ferrites xrd

24 October 2023 7,485 5 View

How lattice constant decrease but crystalline size increase in Mg substituted Ni Zn ferrites?

in Mg substituted Ni Zn ferrites ho Mg effects its structural properties ,dielectric properties, electrical properties .uv spectroscopy

23 October 2023 874 0 View

How crystalline increse in mg substituted Ni Zn ferrites?

crystalline increase as Mg concentration increase

22 October 2023 8,946 0 View

What is difference between dielectric loss and tangent loss in soft ferrites?

both have same graph at low and hihg frequenc

21 October 2023 8,802 2 View

Working principle of vltra visible spectroscopy in mg substituted ni zn ferrites?

working principle of uv

21 October 2023 4,348 0 View

Why ultra visible spectroscopy says?

visible spectroscopy

21 October 2023 6,852 3 View

The reason for breeding Loxp mice with FLPe mice?

I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...

16 July 2024 7,475 1 View

Why do men so often treat their female partners with so little respect?

So what turns her on? … A man who makes her feel ‘safe’ and treats her like she is a vital part of his very existence, and not just a favourite sperm dumpsite. (Yangki Akiteng)

22 June 2024 4,988 2 View

What is the concentration in mL of glycerol and Equex Paste is required to dilute semen to a concentration of 1 x 10^6?

The semen concentration will then be diluted with fraction B (9% glycerol and 1.5% Equex Paste 13560-0008) at 50 °C to a final concentration of 1 x 106 spermatozoa/mL.

23 May 2024 9,071 0 View

What role do epigenetic modifications play in male infertility?

Can epigenetic modifications affect various aspects of sperm development, function, and fertilization?

27 April 2024 6,902 2 View

What are the primary causes and potential solutions for sperm count and shape imbalances ?

How do these abnormalities impact male fertility?

02 April 2024 935 2 View

Can the abnormal sperm(morphologically) fertilize the oocyte ?

Abnormal sperm-morphology

24 February 2024 7,725 6 View

How long to treat male mouse to affect the offspring ? When is the paternal programmation efficient ?

My question is mostly about how many spermatogenesis cycle does it take so all the spermatozoa are effected by a deficiency / treatment, and so the offspring is completely affected ? No matter if...

10 January 2024 7,099 0 View

What is the simplest method for handling epididymis of male albino rat for taking asemen sample for measuring sperm count using hemocytometer?

need steps, the used diluent, dilution factor and the equation.

20 October 2023 8,927 1 View

Problem in goat semen post-thaw sperm motility?

Hi everyone, I am working on goat semen using quercetin as an antioxidant but not getting proper post-thaw sperm motility (40 to 60%). Sperm wave motion above 4 and sperm motility more than 80%....

02 October 2023 5,174 11 View

Am unable to obtain good yield of RNA from mouse spermatozoa. Could anyone help me out to obtain good RNA yield for sequencing ?

I am trying to isolate RNA from mouse spermatozoa to proceed ahead with sequencing but i am unable to obtain good yield of RNA either with Trizol or with RNeasy plus universal mini kit. I have...

16 August 2023 8,658 4 View