Fluorescent tyramide protocol ?

Hello, Im asking to check if anyone has a protocol for preparing fluorescent tyramide for fluorescent in situ hybridization. The one Im using isnt working, so if anyone has one that works, can you...

05 November 2020 9,566 3 View

How to analyse hydrophobic organic cpds in animal fat tissue?

Am looking for the best method to extract and analyse water insoluble organic cpd from animal fat tissue

12 October 2020 1,586 2 View

How do I run a western blot following co-ip?

This is the first time I do any sort of IP experiment and I was wondering if there is a certain procedure to follow for the western blot after the last elution step in the co-ip protocol. I am...

22 September 2020 3,688 3 View

How to lysis an expressing a membrane protein using a plasmid?

I have created a plasmid for my protein of interested tagged with Flag tag. The sequence looks fine but for the life of me I cant get this protein to express itself and see it using western blot (...

30 June 2019 7,043 15 View

Unbalanced data in linear and nonlinear mixed model ?

when fitting a nlme on nested repeated measurements data , models can be unstable if sample sizes across groups are highly unbalanced. In my dataset I have 4 unbalanced groups in terms of...

24 June 2019 1,698 4 View

I am looking for research topic ideas for a systematic review related to electronic medical records?

There are systematic reviews that have explored the barriers and facilitators that influence the success of EMR adoption. I am looking for other research topic ideas for a systematic review...

22 May 2019 9,512 3 View

Methods of unmasking for IHC-P ?

I am trying to calibrate an antibody which on its data sheet specifies it works for parafin IHC. On the datasheet it says it should work with PH-6 HIER method is recommended. I have used Sodium...

13 May 2019 7,706 2 View

Is soxhlet extraction a reliable way of getting pure THC from cannabis plant?

I have been having isssues getting pure THC form the USA and it seems the price from sigma is exhorbitant. I want to get the plant and extract THC. i was told by someone that soxhlet extraction...

15 August 2018 8,300 9 View

What is the best facial expression detection algorithm?

I need the best algorithm for facial expression which can be used with MATLAB.

02 August 2018 7,843 3 View

How can i adjust parameters in logistic regression?

26 February 2016 1,440 13 View

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02 March 2021 3,060 3 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

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22 February 2021 9,932 11 View