Dear All,
We performed some experiments and used the values of absorbance at 570 nm and 600 nm to calculate what the company calls "percentage of reduction". However, with the long time incubations (over 22 hours) we got values with over 100% (111%- 134%). Since these seems to be off, we have checked manually our code and verified that every value was used correctly. As we looked at the formula we noticed that it is measuring a ratio value, but not against a absolute reduced value. See snapshot attached. Are we interpreting it right and the "percentage" given by the companies is wrong?
Any help will be greatly appreciated.
P.S. Because of copyrights I cannot give you a full snapshot of the values.For the full equation please look under "AlamarBlue percentage reduction"