In-house ELISA too much signal. Any suggestions ?

09 November 2020 5,509 5 View

Is this journal a serious journal?

Hello everyone, I want to know your opinion about: "journal of musculoskeletal and neuronal interactions" is it a serious and real journal (not a predatory one) to publish in? Thanks

25 July 2020 3,806 4 View

What is the best solvent/method to be use to prepare PVB (Polyvinyl butyral) solution?

I would like to spin-coat a thin film of PVB - the PVB is in powder form - which solvent should I use to prepare the PVB solution and what are the process to be followed? Thanks

04 February 2019 8,677 15 View

FMRI between-group correlation study: how to specify study variables?

I am designing an fMRI study for my dissertion and I got consfused as I got to this part. Since, I have to signify the study variables one by one in a chart and provide theoritical and operational...

20 January 2019 7,607 9 View

Could anyone tell me how to find refractive index of SiO2 thin film, from its UV/Vis transmission spectrum?

I have recorded UV/Vis transmission spectrum of SiO2 thin film deposited on glass. I want to find the refractive index of the film. My transmission spectrum does not represent interference...

01 June 2018 5,500 5 View

I tried several times to get an ethanolic extract for a plant powder but the extract was very little after evaporating ethanol?

I tried several times to get an ethanolic extract for a plant powder but the extract was very little after evaporating ethanol? what is the reason

06 February 2018 3,462 9 View

Is remote sensing data an alternative of field readings?

The use of remote sensing for the assessment of a aquatic resources provides several advantages compared to conventional approaches for water quality assessments. So, can we replace the field data...

04 February 2018 3,269 31 View

Where do I find data on global mean annual Lake surface temperature?

01 March 2017 977 3 View

Does chemotherapy affect RNA quality and quantity after its extraction from tissues?

15 January 2017 5,413 2 View

How to reconstruct high dimensional space from low dimensional space?

22 March 2016 9,168 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View