Hi everyone, can you suggest a reference to learn RAPD band scoring and statistical analysis?

More Sharon Kadirvelu's questions See All

Can anyone help me find a protocol for broth microdilution method to determine MIC using INT as indicator?

I am doing MIC for few plant extracts by Microdilution method on 96 well plate. I am looking for the protocol for the same which uses INT indicator dye. Also, wanted suggestion on the indicator...

02 March 2018 8,012 2 View

Is there a standard method to extract all the phytochemicals into one solvent, or does it have to be extracted in different solvents?

Dear all, I need to extract phytochemicals from plants to determine it's HPTLC fingerprint. From the papers that I have read, it looks like there are many different methods to extract...

04 May 2016 8,417 30 View

A set of primers is showing the same amplicon in different samples. Is there a way to find out if it is contaminated or some other reason?

03 April 2015 7,546 8 View

Can Tris-Cl be Substituted in place of MES in the vir gene induction medium?

Hi Everyone, I am currently trying to standardize a protocol for Agrobacterium mediated gene transfer in plants. As a crucial step, induction of vir genes in Agro is mandatory and the composition...

02 March 2015 5,613 5 View

Does Oligo dT ensure only mRNA is getting amplified and not genomic DNA contamination?

I am working with aloe vera RNA and have been using the Trizol method of extraction of RNA. I have recently started treating my RNA with DNase (Himedia) (1U/microlt for 1Microg RNA). However, I...

04 May 2014 1,376 8 View

How can I separate almost identical sized DNA mixtures on agarose?

A PCR amplified cDNA sample has been giving me a mixed DNA profile upon sequencing. My sample might be a DNA mixture. When run on 1.5% TAE Agarose gel at 90-100volts, covering almost 3/4th the...

03 April 2014 3,087 14 View

Re-amplifiation of PCR products- High molecular weight smears, absolutely no bands/ primer- dimers

I successfully re- amplified my band of interest once, using 1:1 dilution and increased annealing temperature by 2 degrees. I tried reproducing the same now, its giving me high molecular weight...

04 May 2013 5,949 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View