Sharon Kadirvelu

8 Questions 14 Answers 0 Followers

Questions related from Sharon Kadirvelu

Hi everyone, can you suggest a reference to learn RAPD band scoring and statistical analysis?

I have understood till the PCR part, but not sure how to proceed further to build a phylogenetic tree, which I guess requires statistical analysis. Please help. Thank you

07 July 2018 6,747 5 View

Can anyone help me find a protocol for broth microdilution method to determine MIC using INT as indicator?

I am doing MIC for few plant extracts by Microdilution method on 96 well plate. I am looking for the protocol for the same which uses INT indicator dye. Also, wanted suggestion on the indicator...

03 March 2018 8,092 2 View

Is there a standard method to extract all the phytochemicals into one solvent, or does it have to be extracted in different solvents?

Dear all, I need to extract phytochemicals from plants to determine it's HPTLC fingerprint. From the papers that I have read, it looks like there are many different methods to extract...

05 May 2016 8,481 30 View

A set of primers is showing the same amplicon in different samples. Is there a way to find out if it is contaminated or some other reason?

Dear All, I have been working with a set of primers which was designed a particular cDNA for over a year. For over few weeks now, I find the same amplicon size (0.4kb) in any and every sample;...

04 April 2015 7,610 8 View

Can Tris-Cl be Substituted in place of MES in the vir gene induction medium?

Hi Everyone, I am currently trying to standardize a protocol for Agrobacterium mediated gene transfer in plants. As a crucial step, induction of vir genes in Agro is mandatory and the composition...

03 March 2015 5,699 5 View

Does Oligo dT ensure only mRNA is getting amplified and not genomic DNA contamination?

I am working with aloe vera RNA and have been using the Trizol method of extraction of RNA. I have recently started treating my RNA with DNase (Himedia) (1U/microlt for 1Microg RNA). However, I...

05 May 2014 1,444 8 View

How can I separate almost identical sized DNA mixtures on agarose?

A PCR amplified cDNA sample has been giving me a mixed DNA profile upon sequencing. My sample might be a DNA mixture. When run on 1.5% TAE Agarose gel at 90-100volts, covering almost 3/4th the...

04 April 2014 3,147 14 View

Re-amplifiation of PCR products- High molecular weight smears, absolutely no bands/ primer- dimers

I successfully re- amplified my band of interest once, using 1:1 dilution and increased annealing temperature by 2 degrees. I tried reproducing the same now, its giving me high molecular weight...

05 May 2013 6,045 6 View