Hi everyone, can you suggest a reference to learn RAPD band scoring and statistical analysis?

I have understood till the PCR part, but not sure how to proceed further to build a phylogenetic tree, which I guess requires statistical analysis. Please help. Thank you

06 July 2018 6,695 5 View

Is there a standard method to extract all the phytochemicals into one solvent, or does it have to be extracted in different solvents?

Dear all, I need to extract phytochemicals from plants to determine it's HPTLC fingerprint. From the papers that I have read, it looks like there are many different methods to extract...

04 May 2016 8,417 30 View

A set of primers is showing the same amplicon in different samples. Is there a way to find out if it is contaminated or some other reason?

03 April 2015 7,546 8 View

Can Tris-Cl be Substituted in place of MES in the vir gene induction medium?

Hi Everyone, I am currently trying to standardize a protocol for Agrobacterium mediated gene transfer in plants. As a crucial step, induction of vir genes in Agro is mandatory and the composition...

02 March 2015 5,613 5 View

Does Oligo dT ensure only mRNA is getting amplified and not genomic DNA contamination?

I am working with aloe vera RNA and have been using the Trizol method of extraction of RNA. I have recently started treating my RNA with DNase (Himedia) (1U/microlt for 1Microg RNA). However, I...

04 May 2014 1,376 8 View

How can I separate almost identical sized DNA mixtures on agarose?

A PCR amplified cDNA sample has been giving me a mixed DNA profile upon sequencing. My sample might be a DNA mixture. When run on 1.5% TAE Agarose gel at 90-100volts, covering almost 3/4th the...

03 April 2014 3,087 14 View

Re-amplifiation of PCR products- High molecular weight smears, absolutely no bands/ primer- dimers

I successfully re- amplified my band of interest once, using 1:1 dilution and increased annealing temperature by 2 degrees. I tried reproducing the same now, its giving me high molecular weight...

04 May 2013 5,949 6 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View