Does anyone know the ideal cell density for establishing TIB-75 xenograft in BALBc mice?

I am trying to establish TIB-75 cell line xenograft in BALBc mice.

19 January 2021 5,813 2 View

How is the power spectrum of the backscattering produced by the atmosphere on radar transmission?

If a radar send an electromagnetic wave to track some object I would expect that the returned wave (from the object) is accompanied by backscattering from atmosphere molecules. My question is, how...

17 January 2021 1,208 2 View

QPCR considerations in analyzing samples from the field?

I am attempting to plan a qPCR experiment in which I evaluate the expression of multiple genes in various brain regions of fetuses, as a function of location in utero (intrauterine position,...

31 October 2020 5,788 1 View

Can I perform immunohistochemistry on tissue after it has already been flash frozen?

I did not plan on staining but now it is a necessity. So tissue was immediately frozen using liquid nitrogen and then placed in -80 C after removal. This is human tissue and I would like to stain...

08 October 2017 4,357 3 View

What is the best way to evaluate that the AZD-7762 inhibitor is working on Chk1 upon DNA damage?

Hi! I am trying to inhibit Chk1 kinase phosporilation upon DNA damage. I was thinking on using the inhibitor AZD-7762. What is the best way to evaluate that the AZD is working? Should a western...

05 January 2016 1,464 3 View

Any advice for total protein quantification from cells suspended in FBS?

11 December 2015 9,041 3 View

Why is there no separation in PFGE?

I tried to use PFGE system CHEF II (Bio-Rad), but even on length marker (also Bio-Rad) I cannot receive any DNA separation. I used 0,5X TBE buffer, certified molecular biology agarose from...

13 May 2015 4,614 14 View

What are the best data mining tools for health care data?

We have moved into the era of "big data", and tools that have traditionally been applied to other industries are now being considered in health care. Data sources include claims data, survey data,...

24 March 2015 3,170 11 View

Are there any methods to isolate microorganism which can not grow on standard agar plates?

Hi, I'm lookong for methods for the isolation of microorganisms from liquid consortium, which could not grow on standard, solidified agar. Strains which grow on agar plates are unable to degrade...

17 November 2014 2,572 10 View

Why am I getting no PCR products in long range PCRs using cDNA as template?

08 June 2014 3,011 14 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View