What are the Hypotheses can i frame for the topic " Social media as a Source of Information to Engineering Students"?

i am planning to do research on the topic " Social media as a Source of Information to Engineering Students" for this please share valuable thoughts.. which type of Hypotheses can suitable for...

27 October 2018 8,864 3 View

Can okra plant be propagated by stem cuttings?

Dear All, I have identified a putative male sterile line in okra(Abelmoschus esculentus- through wide hybridization) (confirmed sterility with crossing/ selfing/ pollen studies). In order to...

10 October 2017 7,706 3 View

Can you suggest alternate chemical for potassium tetraborate k2b4o7 which is essential for chitinase assay?

Can you suggest alternate chemical for potassium tetraborate k2b4o7 which is essential for chitinase assay?

20 December 2016 2,182 3 View

Clone having repetitive sequence insert and its discripancy upon scale up?

I have cloned my 3.3Kb insert having repetitive sequence in yeast expression vector, by directional cloning in E.coli. Positive Clone of 8.7Kb was confirmed by restriction analysis. While the same...

26 January 2016 4,477 2 View

How can I implement a indirect matrix converter controller?

electrical engineering

11 February 2015 6,989 3 View

What are the strong reduction agents to remove the epoxy(=O) groups and -COOH groups?

what are the different reducing methods and reduction chemicals...

24 August 2014 2,993 3 View

Comparison of Nano drop and RNA integrity number (RIN) data?

I have isolated total RNA from mammalian cell culture samples. I used a Qiagen Kit. I have checked the conc and quality of RNA by Nanodrop. Ratio of 260/280 was between 2.01 and 2.09, and 260/230...

21 August 2013 4,011 16 View

mRNA level and its comparison to protein level.

I am measuring a gene which is involved in differentiation of a cell. During differentiation its level is high, compared to undifferentiated state. Upon terminal differentiation of cell, mRNA...

20 June 2013 2,588 2 View

suggestions for using alternate pcr method for race

hello friends! any one suggest me alternate pcr method for plant cloning and expression instead of using race..

25 July 2011 3,681 1 View

Chromosomal counting in okra species?

I have been trying to prepare root tips for chromosome counting studies in okra (Abelmoschus), but every time I am failing to get stained root tips with Feulgen stain. The root tip portion is not...

01 January 1970 5,105 4 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

How do you prepare a porous crosslinked Polyvinyl alcohol and Glutaraldehyde membrane or film?

I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption

08 February 2021 7,493 3 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View

Why am I getting such large heats of dilution in my Isothermal Titration Calorimetry Experiment?

I am trying to measure binding of calcium to an expressed protein we are studying and am getting very large heats of dilution for the calcium solution in my control tests. We prepared a 1 M CaCl2...

07 February 2021 7,532 1 View

Acoustic simulation of Membrane?

I have 1 mm thick membrane. This membrane is perforated. I want to simulate this in Acoustic module of comsol. For this ,which type of module I can use in comsol.?

02 February 2021 2,505 6 View