I am planning to develop a Human T cell line having the potential to kill OVA expressing tumor lines.
Mostly we have to take out Tcells from OT1 mice which is expensive in overall cost.
If I can, kindly suggest the best plasmid for the same.
Or can I plan immortalization of OT1 mice T cells?
So that I will not need to sack the mice again and again.
Which of the task will be more feasible..