Vikas Somani

4 Questions 2 Answers 0 Followers

Questions related from Vikas Somani

HI, Can I engineer any immortal human T cell line with the murine TCR of Ovalbumin ? Just like OT1 mice T cell have the same receptor ?

I am planning to develop a Human T cell line having the potential to kill OVA expressing tumor lines. Mostly we have to take out Tcells from OT1 mice which is expensive in overall cost. If I...

12 December 2019 8,303 2 View

How to confirm presence of antibody in mouse tissue sample ? I am looking for antibodies in subcutaneous tumour tissue sample ?

Detection of Presence of specific antigen in fixed or fresh tissue sample is common task. How to detect presence of ab (IgG or IgM ) in a fixed or fresh tissue of subcutaneous tumour ? IHC or...

07 July 2019 2,628 3 View

What are the best programe and prediction tools for subcellular localisation of bacterial protein ?

Any software and tools linked with the database for gram positive bacterial transmembrane protein like spfh domain protein.

09 September 2014 5,602 5 View

How can I separate large size plasmid (200kb) from gram positive bacteria bacillus anthracis? .

If we isolate genome, then plasmid comes together. Even all the plasmid isolation procedure showed presence of genomic dna also.

01 January 1970 4,250 2 View