Hi can anyone suggest me from where to get Uveal mealanocyte cell line?

09 September 2018 5 1K Report

I am looking for Uveal mealnocyte cell line source as a control for uveal mealnoma cell lines.

ATCC offers three different cell lines derived from uveal (eye) tissue for melanoma indication. Two of these cell lines are indicated by catalog numbers MP38 (ATT CRL-3298) and MP41 (ATCC CRL-3297).

Hope this helps.

Anil Tiwari

Thanks Eric, but these cell lines are uveal melanoma, tumor cell line. I'm looking for uveal melanocyte cell line, not tumor one.

Amos Bairoch

ATCC used to distribute PIG1:

https://web.expasy.org/cellosaurus/CVCL_S410

which was HPV16 transformed melanocyte cell line.

But they discontinued that product. Why I have no clue!

A Chinese group developed a melanocyte cell line, UM-U-95:

https://web.expasy.org/cellosaurus/CVCL_RY60

But its not weel characterized and was only described and used in one publication.

There is also a very old report from 1980 on the U1I cell line:

https://web.expasy.org/cellosaurus/CVCL_IW88

But that looks also like a one shot!

Trying to see what happened to PIG1, I found out the group has also developed an other HPV16 immortalized melanovyte cell line: PIG3V which I will add at the next release of the Cellosaurus:

ID PIG3V

AC CVCL_VR17

RX PubMed=10937834;

CC Doubling time: 1.6 days (PubMed=10937834).

CC Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16) [E6/E7 (pLXSN)].

DI NCIt; C26915; Vitiligo

OX NCBI_TaxID=9606; ! Homo sapiens

SX Female

AG 34Y

CA Transformed cell line

Maybe you should contact Isabelle C Le Poole, the first authors on the PIG cell lines:

https://www.feinberg.northwestern.edu/faculty-profiles/az/profile.html?xid=38353

Good luck and if you do manage to get one, please feedback the info!

Amos Bairoch

Anil Tiwari

Thanks a lot Amos Bairoch for your detailed reply and information. I appreciate you time and efforts.

Thanks

Anil

Leo Urmaza

Hello Anil,

I am not sure if they have it but you can check and inquire with ABM (www.abmgood.com). They have a collection of cell lines available in the market.

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