Hi can any one plz send me protocol of DNA extraction of pine tree ?

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When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,284 4 View

How to delete my paper?

i want to delete my research paper.

09 July 2024 6,554 2 View

Is it okay to use reviewed proteins?

I'm working on the proteome (from Uniprot), as you're probably aware that there are reviewed and unreviewed proteins, however unreviewed proteins are in large quantities, but reviewed proteins are...

09 July 2024 5,782 2 View

Does anyone know what to do if Horiba ABX pentra 400 won't run positive and negative controls for LDH?

I am trying to run LDH assay on my samples using Hroiba ABX pentra 400. Multical has worked but negative and pos control are showing an error message saying no control in memory (image attached)....

18 June 2024 6,514 0 View

We are currently compiling the results in network pharmacology. Could you please assist me with this?

I have nearly completed my preparation for the short communication. However, I could use some assistance with the results. Any help would be greatly appreciated.

12 June 2024 4,230 1 View

How to calculate sample size for Quasi-Experimental Design Research?

21 May 2024 9,555 3 View

Floods around the world and their general characterization research papers?

suggest research regarding this topic

17 May 2024 9,366 0 View

Literature Review chapter consists of sub-headings?

Some people suggest that the introductory chapter of the dissertation should consist of subheadings compared to the literature review chapter? Can anyone please confirm which is more acceptable?

15 May 2024 4,037 1 View

What is the difference between conceptual framework and working model while writing the dissertation?

12 May 2024 6,306 1 View

An engineer wants to use a circular metallic section, in a portion of a building where the torsion demand is high. Recommend a suitable metal among?

Perform Experiment and report

11 May 2024 3,449 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View