Cytoskeleton remodulation !
I attempted a quick test to generate the .gro file from the .pdb file, which I initially copied from our main source. I obtained this error: "Atom OA in residue MET 1 was not found in rtp entry...
17 March 2024 8,970 0 View
Hello. I am looking for a cell line with similar properties to human Dental Pulp Stem Cells. DPSCs have the ability to differentiate into odontoblasts in culture. All commercially available cell...
03 June 2021 7,295 0 View
I have been trying to conduct the ansidine value experiment (AOCS official method Cd 18-90) for quite a while now but the values I am getting are much higher than they should be. For example, for...
12 June 2018 6,976 6 View
Can anyone explain the pictures? please open the attachment file what is the deference between these pictures one did by Scanning electron microscopic (A) and another did by Transmission electron...
24 August 2016 2,532 3 View
Hello guys, i have been working on this problem for quite some time now, i was able to plot Nu vs theta using poly lines,expressions and variables however the plot does not start from 0 degrees...
11 February 2016 396 6 View
And if yes, what is the relation between them? In the first phase of chloride-induced corrosion, i.e. diffusion of chlorides, chlorides are generally considered as the measured criteria to...
20 August 2015 9,880 5 View
What type of cells can be measured using the Cell Counting Kit-8? Is pre-incubation necessary when using the Kit-8? and if so, How much pre-incubation time is required prior to the assay? What...
26 December 2014 4,903 10 View
How to benefit from Distributed Ledger Technology(DLT) + Machine Learning /Deep Learning for the detection of COVId-19 Virus?
01 January 1970 7,416 2 View
Hello, I am working on a clinical project with two groups: monophasic and relapsing patients. The number of patients in each group is not the same. n = 28 for monophasic group and n = 15 for...
01 January 1970 6,380 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View
