Hei, at my hospital the flow Department always ask the material for flow be sent in lithium heparin.
Is there any problem sending the material With natrium heparin?
thanks!
For the analysis of the immunophenotype of lymphocytic subpopulations, the sample to perform this procedure is whole blood and to preserve its integrity, it is recommended to collect the sample in anticoagulant tube EDTA-K2, or EDTA-K3 instead of sodium heparin, due to the use Prior to the hematological counter and not to alter the conformation of the antigens to be studied. For the analysis of the immunophenotype of Acute Leukemias by CMF, the same criteria is recommended. For the determination of platelet activation antigens and leukapheresis products, it is recommended to use citrate dextrose acid (ACD) and phosphate citrate dextrose (CPD), respectively.
In those samples constituted by unicellular dispersions of solid tissues; For example of ganglion BAAF, it is desirable that the anticoagulant is diluted in up to 1 mL volume of a sterile isotonic liquid (physiological saline or phosphate buffer with pH 7.4) filtered by filters with a pore of 1 cm), it is advisable to cut it with a scalpel in smaller pieces of a few mm3.
genail Juan Carlos, yo mando solo medula osea, por lo que entendi EDTA es mejor que cualquier heparina?
sabes porque me piden mandar lithium heparin y no sodium ? (la verdad es que les mande sodium heparin varias veces y el resultado me vino perfecto....)
no se si ponen la muestra en el celldyn antes de analizarla, la citometria esta en inmunologia y creoq q no tienen contadores
Un saludo
Cecilia
Regarding the differences between Li- and Na-heparin we must consider that the salt (Li or Na) have no effect anti-coagulant, because this is only caused by heparin. Thus, as regards the differences in use between EDTA and heparin, I agree completely with Juan Carlos, instead the differences between LI-Na should not be significant for the use requested by Maria Cecilia.
many thanks , I thought so, as I sent some specimens With na-heparin and they were processed without a problem
For flow cytometry studies, we have used EDTA instead of heparin for better stability of cells. In some specimens with heparin we have had problems of microcoagulation and aggregation of platelets .
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