The steam explosion has been used for a pretreatment of poplar wood based ethanol production in our recently research. The hemicellulose content of samples were analysed using the American Renewable Energy Laboratory standard method. However, the contents of cellobiose and arabinose were below the detection limits.Could anyone tell me some reason of this result? Is it suitable to calculate the content of hemicellulose only use the xylose detected?
Mannan is second most abundant hemicellulose after xylan so you can measure mannan content. Mannan is polymer of mannose and could be detected by same method which was you have used for xylose detection.
The main sugar component of hemicellulosic fraction of poplar is xylose. Please find attached the review paper from Ragauskas et al. which clearly confirms the same. In that case you can calculate the xylan content based on xylose concentration only
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