Hello everyone, I am currently doing my research project for my final year of degree and I don't know how to go about the western blot. I have never done this technique before and my supervisor doesn't help me either, he is so vague and not in the lab 90% of the time. What I am trying to do it check if our protein of interest is present in the neutrophils. So i know how to extract the neutrophils but don't know how to go on further. My supervisor doesn't have any post-docs or phD under him, so I don't have anyone to ask in the lab apart from post docs in other groups, but they are always busy with their own work. My supervisor ordered the Antibody for the protein, now i don't know how to go about this. Does anyone have a protocol that is easy to follow? any advise would be greatly appreciated. I am hating my project now :(