Help with MPO (myeloperoxidase) ELISA?

15 April 2020 0 5K Report

Hi all.

I have done MPO ELISA's in past on cell pellets and serum samples. This time I have whole blood and spleen homogenates as samples. I did some research online and for the spleen homogenate it seems i just have to thaw the samples, centrifuge at 10000 rpm for 10 minutes and proceed with the assay. Does it sound right?

Also, i could not find any info on whole blood as samples. Currently, the blood is at -80 in an anticoagulant. Do i lyse it with some buffer after thawing or i straightaway pick the volume for the assay?

These samples were collected from mice depleted for PMNs and we are tracking the timevwhen PMNs return in blood and spleen.

I would appreciate any input.

Regards

Manmeet

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