Hello everyone,
I need to use a final concentration of 90ng/uL of BSU polymerase in a final volume of 50uL. The NEB BSU polymerase specification sheet indicates 5ng/uL as the stock concentration. How do I convert units/uL to ng/uL knowing that one unit is defined as the amount of enzyme that incorporates 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.
I am not 100% sure but I know that:
Specific activity (units/ng) = 5 U/μl / 10 nmol
To convert nmol to ng, we need to know the molecular weight of dNTP. Assuming an average molecular weight of 330 g/mol for a single nucleotide, we can calculate the specific activity in units/ng as follows:
Specific activity (units/ng) = 5 U/μl / (10 nmol x 330 g/mol)
and... if this is correct... then what?
Thanks a lot :)