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Questions related from Claudia Nicolini
Hi :) How should I design primers for (-)ssRNA samples from Influenza A and hRSV viruses? I need to select the protein, locate the gene sequence, and then use it to design primers. Should I...
05 April 2024 4,779 1 View
Hi everyone, Can you help me clarify what are the steps to amplify an RNA extracted from a virus? what are the essential steps to amplify viral RNA? Does incubation with a reverse...
21 March 2024 9,758 0 View
I studied the effect of the crowding agent on recombinase polymerase amplification using the TwistDX kit. I prepared the rehydration buffer in house following the company's patent table and...
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Does anyone have any experience with the Nickase Strand Displacement Amplification technique? I am trying to figure out how to design the primers but I need some help :) DOI:...
26 February 2024 9,606 0 View
Hello everyone, I need to use a final concentration of 90ng/uL of BSU polymerase in a final volume of 50uL. The NEB BSU polymerase specification sheet indicates 5ng/uL as the stock concentration....
07 February 2024 5,858 2 View
I have read in several articles that it could be an enhancer of PCR efficiency. Is this the case? How and why?
07 February 2024 5,993 3 View
We usually use Excel in our lab to analyse data, but I would like to take a course on a more sophisticated tool. Can you share which one is most common in molecular biology? Thank you :)
29 January 2024 8,490 11 View
I’d like to do it in house and figure out which reagent makes the solution so viscous, it’s not good for a fluidic methods. I would like to know if anyone tries anything different to resuspend...
12 December 2023 9,858 0 View
