Can we use a compensation matrix with single staining that the negative population for each single staining is below zero?

Recently we used single staining to make a compensation matrix. But, for each single staining we saw that 13 to 25 percent of our negative population had been shown below Zero. In this situation...

21 February 2021 9,982 2 View

What is the best studying timeline after injection of B16-F10 melanoma metastases cell line?

I want to inject B16F10 cells into C57BL/6 mice as a melanoma metastases model to study the speed of metastases in different organs. I need advice about the designing of the best timeline for...

14 January 2021 3,704 1 View

How to change the atom numbers of a residue in a pdb file?

I am trying to dock 2 proteins in HADDOCK server, but it repeatedly shows the following Error for the second pdb file: " Error in PDB file. Your PDB contains multiple residues with number 1 in...

25 November 2020 4,753 1 View

From where I can get hourly precipitable water vapor (PWV) data for a location?

Hello, For completing my model for radiative sky cooling, I need the precipitable water vapor (PWV) data for a particular location in order to estimate the atmospheric emissivity. Unfortunately, I...

14 September 2020 343 7 View

I am looking for a job as a solid state chemistry researcher in Germany. Could anybody help me in this regard, please?

I'm PhD graduated in Inorganic Chemistry. Currently I am searching for a new position in metal-organic frameworks (MOFs) & li-ion batteries as a Postdoc or Ph.D. researcher. You can find my...

05 April 2019 2,541 1 View

Which image processing software is the best for gel densitometry?

I am using the "ImageJ" software in order to quantify the protein bands on the SDS-PAGE, but I'm not sure it's the best software for this purpose. Does anyone have the experience of using other...

10 January 2019 2,882 12 View

How to analyze SRB ( Sulforhodamine B ) assay results?

I am working on melanoma B16-F10 cell line and after treatment whit different concentration of drug I do SRB protocol then I read their absorb at wavelengths of 545 nm. now, how to analyze these...

12 December 2018 9,843 2 View

What is the best method to dilute DNA samples?

I want to create a serial dilution of genomic DNA and plasmid samples for absolute qPCR. In order to get repeatable results in the Real time PCR, I want to do a very accurate dilution. I know that...

25 October 2018 5,282 12 View

Does anybody have a Matlab code for LDPC encodong and decoding for a rate other than 1/2?

Hi, does anybody have a matlab code for LDPC encodong and decoding for a rate other than 1/2? My message length is not very large.

05 August 2018 9,338 3 View

Why rust artificial inoculation did not work ?

20 May 2018 4,548 7 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View