Hello Dear Scientists? Phytochemicals VS Synthetic Chemials? Which one better and Why?

Dear I am working on obesity and writing a review article. I want to add the topic that Why Natural products (Phytochemicals) are more beneficial than synthetic ones? I know that they have less...

02 March 2019 693 2 View

Does filteration effects the crude extracts activity?

Dear Scientists, I applied the pure crude extracts (without filtration by syringe filter) on colon cancer cell lines and got some good results. When I repeated the experiment with filtration by...

10 November 2017 6,844 0 View

No title

I have done the extraction with 90% Aqueous Ethanol of 25 gms of the sample and then filtered and then evaporated the solvent. After this, I did Lypholizations and after lyophilization, I got 0.5,...

06 July 2017 6,745 0 View

Sticky sample after Lypholization

Dear scientists, I lyophilized my plant samples and after lyophilization, for some sample, I got a very good fine powder but for some samples I got a sticky solid form just like gum(Not fine...

06 July 2017 5,837 0 View

Total Triterpenoid determination.?

04 May 2017 3,628 3 View

Request for articles

hello all, Can any one suggest or send specific articles for quantitative analysis of Triterpenoids (Taraxasterol), by using C18 Column in HPLC-ELSD method ?

02 March 2017 6,830 0 View

Request for Article for (Triterpenoids) taraxasterol and its actate HPLC analysis??

hello every one. Can anyone send an article in which method of gradient elution for triterpenoids like taraxasterol and its acetate has been analysed by HPLC by using different gradient...

02 March 2017 9,122 3 View

Important Question

Dear Scientists, I am confused now. I did extraction from plant with 90 percent ethanol at 40 degree centigrade in reflux condensor, then i evaporated the ethanol in evaporator and also in vaccum...

01 February 2017 1,921 3 View

Important questions request about HPLC

For HPLC analysis of Phenols and Triterpenoids, what concentration of standards and samples solutions (In mg/ml or microgram /ml) for injection into HPLC should be used? I just want to know about...

01 February 2017 5,382 4 View

Hello to all scientists. ?

Can any one provide me some good articles for the determination of Triterpenoids by HPLC-ELSD methodology?

31 December 2016 284 0 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View